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KMID : 0380020030180040312
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 4 p.312 ~ p.317
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Effect of Glycine Supplement on Extracellular Secretion of Levansucrase from Pseudomonas aurantiaca S-4380 in Recombinant Escherichia coli
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Kim Seung-Hwan
Jang Eun-Kyung
Kim In-Hwan
Jang Ki-Hyo
Kang Soon-Ah
Jang Byeong-Il
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Abstract
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The addition of glycine up to 0.5％ (w/v) to Luria broth (LB) media on the secretion of levansucrase in a recombinant strain Escherichia coli JM109/pUPLK1 was observed to enhance the release of periplasmic proteins from the cell to the broth, without significantly affecting the cell growth rate and protein productivity. However, the glycine concentration at 1 ％ (w/v), the cell density attainable at the stationary phase fell to about 50％ and the extracellular activity of levansucrase corresponded to about 80％ of the total (extracellular plus intracellular) activity and increased by 2.6-fold, comparing to the cells grown in the absence of glycine. The increased pH at stationary phase accelerated the degradation of levansucrase. Maximal extracellular activity was attained when 1 ％ glycine was supplemented at the onset of strain growth.
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KEYWORD
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glycine, levansucrase, extracellular, supplement
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